missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ Silencer™ siRNA Starter Kit
Click to view available options
:
1 kit
Description
Perfect for researchers new to RNAi, this Invitrogen™ kit contains all of the reagents, protocols, and instructions necessary to get underway with siRNA transfection and ensure successful gene knockdown experiments.
The perfect choice for those beginning siRNA experiments
Includes transfection agent, siRNAs, and three different systems for assessing knockdown
For use with human, mouse, and rat cells
The Silencer™ siRNA Starter Kit includes a high-quality, lipid-based transfection agent, siPORT™ NeoFX™, that effectively delivers siRNAs to a broad range of cell lines. It can be used for reverse transfection, a streamlined protocol that eliminates an entire day from the RNAi experimental procedure; it can also be used with standard transfection protocols. This reagent is not sensitive to serum and efficiently transfects low concentrations of siRNA. The kit also includes a well-characterized GAPDH siRNA, which has been shown to strongly down-regulate GADPH in human, mouse, and rat cells, as well as a nontargeting negative control siRNA, Silencer™ Negative Control #1 siRNA. Because GAPDH is ubiquitously expressed and is easy to detect at both the mRNA and protein level, it is an ideal target for a positive control siRNA.
Three Detection Systems
The kit includes three different systems for monitoring GAPDH siRNA-induced knockdown. An anti-GAPDH antibody is provided to assess GAPDH protein levels by western blot or immunofluorescence. The KDalert™ GAPDH Assay is also provided to quickly measure GAPDH enzymatic activity. This assay can also be used to assess cell viability. Finally, RT-PCR primers are included, for real-time quantitative RT-PCR detection of GAPDH mRNA using SYBR™ Green. These reagents can also be used to transfect miRNAs, such as the Invitrogen™ Pre-miR™ miRNA Precursor Molecules.
The Silencer™ siRNA Starter Kit includes a high-quality, lipid-based transfection agent, siPORT™ NeoFX™, that effectively delivers siRNAs to a broad range of cell lines. It can be used for reverse transfection, a streamlined protocol that eliminates an entire day from the RNAi experimental procedure; it can also be used with standard transfection protocols. This reagent is not sensitive to serum and efficiently transfects low concentrations of siRNA. The kit also includes a well-characterized GAPDH siRNA, which has been shown to strongly down-regulate GADPH in human, mouse, and rat cells, as well as a nontargeting negative control siRNA, Silencer™ Negative Control #1 siRNA. Because GAPDH is ubiquitously expressed and is easy to detect at both the mRNA and protein level, it is an ideal target for a positive control siRNA.
Three Detection Systems
The kit includes three different systems for monitoring GAPDH siRNA-induced knockdown. An anti-GAPDH antibody is provided to assess GAPDH protein levels by western blot or immunofluorescence. The KDalert™ GAPDH Assay is also provided to quickly measure GAPDH enzymatic activity. This assay can also be used to assess cell viability. Finally, RT-PCR primers are included, for real-time quantitative RT-PCR detection of GAPDH mRNA using SYBR™ Green. These reagents can also be used to transfect miRNAs, such as the Invitrogen™ Pre-miR™ miRNA Precursor Molecules.
Specifications
Specifications
| Content And Storage | Anti-GAPDH Monoclonal Antibody, GAPDH PCR Primers, KDalert™ Lysis Buffer, KDalert™ Solution A, KDalert™ Solution B, and KDalert™ Solution C should be stored at –20°C. GAPDH enzyme, GAPDH siRNA, and Negative Control #1 siRNA should be stored at below –70°C. siPORT™ NeoFX™ Transfection Agent should be stored at 4°C. Nuclease-free water may be stored at any temperature. |
| Format | Kit |
| Control Type | Negative Control, Positive Control |
| RNAi Type | siRNA, miRNA |
| Sample Type | Synthetic siRNA, miRNA |
| For Use With (Application) | Transfection |
| Label or Dye | SYBR Green |
| Product Line | Silencer, Ambion |
| Product Type | siRNA Starter Kit |
| Quantity | 1 Kit |
For Research Use Only. Not for use in diagnostic procedures.
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?Share a Content Correction