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Applied Biosystems™ Power SYBR™ Green RNA-to-CT1-Step Kit

Brand:  Applied Biosystems™ 4391178

Code : 50

Additional Details : Weight : 0.00450kg

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Product Code. 10734227

  • £162.00 / Each

EU Stock 13

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR™ Green RNA-to-CT1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes:
  • AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
  • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
  • An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
  • RNase inhibitor, to prevent degradation of RNA templates
  • A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments.
  • Specifications

    Specifications

    Sufficient for 40 reactions at 50 µL reaction volume and includes:
    • 1 x 1 mL tube of 2X master mix containing SYBR®Green 1 Dye, AmpliTaq Gold® DNA Polymerase UP, dNTPs, Passive Reference 1, and optimized buffer components. Store at 2-8°C after opening.
    • 1 x 20 µL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase and RNase Inhibitor. Store at -15 to -30°C.


    Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
    ROX (Pre-mixed)
    Unlabeled Oligos
    UP (Ultra Pure)
    Wet Ice
    7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
    Standard
    SYBR® Green
    AmpliTaq Gold DNA Polymerase
    RNA-to-CT™, SYBR®
    ArrayScript™ UP
    SYBR
    1-Step qRT-PCR
    RNA
    Videos
    Documents