I-A/I-E Rat anti-Mouse, Brilliant Violet 711, Clone: 2G9, BD Optibuild™

Rat Monoclonal Antibody

Brand:  BD Biosciences 743874

Product Code. 15808609

Description

The 2G9 monoclonal antibody reacts with the I-Ad and I-Ed MHC class II alloantigens. It also reacts with transfectants expressing I-Eb and I-Ek and with cells from mice of the H-2p and H-2q haplotypes. This antibody is non-reactive with cells from SJL (H-2s) and NOD (H-2g7) mice. Flow cytometric analysis indicates that the 2G9 and M5/114.15.2 monoclonal antibodies have comparable reactivity on cells from mice with I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek alloantigens.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.

Specifications

• Antigen: I-A/I-E
• Clone: 2G9
• Conjugate: Brilliant Violet 711
• Gene Alias: H-2I, Ia Ag, I-Ad, I-Ed, I-Eb, and I-Ek MHC class II alloantigens
• Immunogen: BALB/c mouse epidermal Langerhans cells
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2a κ