Gibco™ Human WISP-3 Recombinant Protein, PeproTech®

Product Code. 17880973

Brand: Gibco™ 12020100UG

Description

Recombinant Protein

120-20-1MG will be provided as 4 x 250 μg (120-20-250UG). 120-20-500UG will be provided as 2 x 250 μg (120-20-250UG). Recombinant Human WISP-3 is a 36.8 kDa protein containing 331 amino acid residues. It is composed of four distinct structural domains (modules): the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cysteine knot-like (CTCK) domain. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

WISP3 is a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. WISP3 is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of the WISP3 gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis.

Specifications

• Accession Number: O95389
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 8838
• Molecular Weight (g/mol): 36.8 kDa
• Name: Human WISP-3
• Quantity: 100 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: CCN family member 6; CCN6; Cellular communication network factor 6; ENSMUSG00000062074; Gm735; LIBC; PPAC; PPD; RGD1564120; UNQ462/PRO790/PRO956; Wisp3; WISP-3; WNT1 inducible signaling pathway protein 3; WNT1-inducible-signaling pathway protein 3
• Common Name: WISP3
• Gene Symbol: CCN6
• Biological Activity: The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 ug/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: TGPLDTTPEG RPGEVSDAPQ RKQFCHWPCK CPQQKPRCPP GVSLVRDGCG CCKICAKQPG EICNEADLCD PHKGLYCDYS VDRPRYETGV CAYLVAVGCE FNQVHYHNGQ VFQPNPLFSC LCVSGAIGCT PLFIPKLAGS HCSGAKGGKK SDQSNCSLEP LLQQLSTSYK TMPAYRNLPL IWKKKCLVQA TKWTPCSRTC GMGISNRVTN ENSNCEMRKE KRLCYIQPCD SNILKTIKIP KGKTCQPTFQ LSKAEKFVFS GCSSTQSYKP TFCGICLDKR CCIPNKSKMI TIQFDCPNEG SFKWKMLWIT SCVCQRNCRE PGDIFSELKI L
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.