Invitrogen™ Human VPS4B (aa 83-112) Control Fragment Recombinant Protein

Product Code. 30212537

Brand: Invitrogen™ RP104038

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (80%), Rat (80%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-63460 (PA5-63460. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16.

Specifications

• Accession Number: O75351
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 9525
• Name: Human VPS4B (aa 83-112) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 8030489C12Rik; cell migration-inducing 1; cell migration-inducing gene 1 protein; MIG1; Protein SKD1; SKD1; SKD1B; Suppressor of K(+) transport growth defect 1; suppressor of K+ transport defect 1; vacuolar protein sorting 4 homolog B; vacuolar protein sorting 4 homolog B (S. cerevisiae); vacuolar protein sorting 4 B; vacuolar protein sorting 4 b (yeast); vacuolar protein sorting-associated protein 4 B; VPS42; VPS4-2; Vps4b
• Common Name: VPS4B
• Gene Symbol: VPS4B
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: AQKPVKEGQPSPADEKGNDSDGEGESDDPE
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining