Invitrogen™ Human Visfatin ELISA Kit

Product Code. 17175269

Brand: Invitrogen™ EH482RBX5

Description

ELISA

Human Visfatin quantitates human Visfatin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Visfatin.

Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.

Specifications

• Accession Number: 1110035O14Rik; AI314458; AI480535; DKFZp666B131; EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; nampta; nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase a; PBEF; Pbef1; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; Pre-B-cell colony-enhancing factor 1 homolog; RCJMB04
• Assay Range: 1.1 to 300 ng/mL
• Assay Sensitivity: 1.1 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 10135
• Gene Symbol: NAMPT
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: NAMPT
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins