Invitrogen™ VEGF Receptor 3/FLT4 Human ELISA Kit

For the quantitative detection of human VEGF-R3/FLT-4.

Brand:  Invitrogen™ BMS2064

Product Code. 15551577

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human VEGF-R3/FLT-4
Biotin-Conjugate anti-human VEGF-R3/FLT-4 monoclonal antibody
Streptavidin-HRP
Human VEGF-R3/FLT-4 Standard lyophilized, 80ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

The Human Vascular Endothelial Growth Factor Receptor 3/FLT4 (Hu VEGFR3) ELISA quantitates Hu VEGFR3 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VEGFR3. Principle of the method The Human VEGFR3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The main function of the lymphatic system is to return plasma back to the circulating blood. Plasma fluid, macromolecules and cells, such as leucocytes and activated antigen-presenting cells, enter the blind-ended lymphatic vessels. Lymph is then transported toward collecting lymphatic vessels and is returned to the blood circulation. Scientific studies over the past years have revealed a signal-transduction system for lymphatic endothelial cell growth, migration and survival. This system is formed by VEGF-C and VEGF-D and their shared receptor VEGFR-3/FLT-4. Homozygous deletion of VEGFR-3 in mice leads to defects in blood-vessel remodelling and embryonic death at mid-gestation, indicating an early vascular function for the receptor. Furthermore mouse studies suggest that upregulation and activation of VEGFR-3 have functional roles in sprouting angiogenesis and that VEGFR-3 is an important effector of the vascular phenotype resulting from Notch inhibition. In lymphoma patients, high expression of VEGFR-3 can been found in lymph endothelial cells isolated from the tumour tissue.

Specifications

• Accession Number: P35916
• Assay Sensitivity: 0.03 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: FLT-41,LMPH1A,PCL,VEGFR-3,VEGFR3,FLT4
• Intraassay CV: 5.7%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 30 min.

• Assay Range: 0.6-40.0 ng/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 2324
• Interassay CV: 5%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Plasma, 20 μL; Serum, 20 μL; Supernatant, 20 μL
• Synonym: FLT-4, VEGF receptor 3, PCL
• Test Time: 1 hr. 20 min.

For Research Use Only