Gibco™ Human VEGF-B Recombinant Protein, PeproTech®

Product Code.	Quantity	unitSize
17860013	100 μg	100µg
17810193	1 mg	1mg
17831033	20 μg	20µg
17850653	250 μg	250µg
17821333	500 μg	500µg
17881503	5 μg	5µg

Product Code. 17860013 Supplier Gibco™ Supplier No. 10020B100UG

Description

Recombinant Protein

Recombinant Human VEGF-B is a 38.0 kDa, disulfide-linked homodimeric protein consisting of two 167 amino acid polypeptide chains. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.

Specifications

• Accession Number: P49765
• For Use With (Application): ELISA, Functional Assay, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 7423
• Molecular Weight (g/mol): 38 kDa
• Name: Human VEGF-B
• Quantity: 100 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: flt-1/VEGFR1 ligand; vascular endothelial growth factor B; vascular endothelial growth factor B167 precursor; vascular endothelial growth factor B186 precursor; vascular endothelial growth factor-B; Vegfb; VEGF-B; VEGFL; VEGF-related factor; Vrf
• Common Name: VEGFB
• Gene Symbol: VEGFB
• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) in the presence of human VEGF 165. The expected ED50 for this effect is 1.0-2.0 ug/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: PVSQPDAPGH QRKVVSWIDV YTRATCQPRE VVVPLTVELM GTVAKQLVPS CVTVQRCGGC CPDDGLECVP TGQHQVRMQI LMIRYPSSQL GEMSLEEHSQ CECRPKKKDS AVKPDSPRPL CPRCTQHHQR PDPRTCRCRC RRRSFLRCQG RGLELNPDTC RCRKLRR
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.