Gibco™ Human TPO (Thrombopoietin) Recombinant Protein

Product Code. 11370842

Brand: Gibco™ PHC9511

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human Thrombopoietin in PBS containing 0.1% human serum albumin. Further dilutions should be made in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA. Storage: Store this lyophilized preparation at <-20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at <-20°C. Avoid repeated freeze/thaw cycles.

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.

Specifications

• Accession Number: P40225
• For Use With (Application): Bioactivity
• Formulation: PBS with no preservative
• Gene ID (Entrez): 7066
• Molecular Weight (g/mol): 85 kDa
• Name: Human TPO (Thrombopoietin)
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: C-mpl ligand; Megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; Mgdf; MKCSF; ML; MPL ligand; Mpllg; MSA; Myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; TDH2A; THCYT1; THPO; Thrombopoietin; thyroid microsomal antigen; thyroid peroxidase; thyroperoxidase; Tpo; TPX
• Common Name: Thrombopoietin
• Gene Symbol: THPO
• Biological Activity: ED50 = 0.5 - 5 ng/mL; determined by dose-dependent proliferation of human TF-1 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Content And Storage: -20°C
• Classification: Carrier-Free
• Activity: ED50 = 0.5 - 5 ng/mL; determined by dose-dependent proliferation of human TF-1 cells.
• Bioassay: Proliferation of TF1 cells
• Endotoxin Level: <1 EU/ml
• Shipping Condition: Dry Ice
• Expression System: HEK293 cells
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: TPO
• Research Category: Endocrinology, Stem Cell Research, Angiogenesis
• Product Line: Gibco
• Purity or Quality Grade: >90% by SDS-PAGE