Gibco™ Human TGF-alpha, Animal-Free Recombinant Protein, PeproTech®

Product Code. 17883063

Brand: Gibco™ AF10016A500UG

Description

Recombinant Protein

Recombinant Human TGF-alpha is a 50 amino acid polypeptide (5.5 kDa), which shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Specifications

• Accession Number: P01135
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 7039
• Molecular Weight (g/mol): 5.5 kDa
• Name: Human TGF-alpha, Animal-Free
• Quantity: 500 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/ μg
• Gene Alias: EGF-like TGF; ETGF; I79_019067; LOC100009150; Protransforming growth factor alpha; RATTGFAA; TFGA; TGF a; TGF type 1; TGF a; TGFA; TGFAA; TGFalpha; TGF-alpha; TGFa; Transforming growth factor; Transforming growth factor alpha; transforming growth factor, alpha; transforming growth factor-alpha; wa1; wa-1; waved 1
• Common Name: TGF alpha
• Gene Symbol: TGFA
• Biological Activity: Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10^6 units/mg
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.