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  5. Invitrogen™ Human TfR/Transferrin R/CD71 ELISA Kit

Invitrogen™ Human TfR/Transferrin R/CD71 ELISA Kit

Product Code. 17164753
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5 x 96 Tests
96 Tests
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Product Code. Quantity unitSize
17164753 96 Tests Pack of 96
17195229 5 x 96 Tests Each
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Product Code. 17164753 Supplier Invitrogen™ Supplier No. EH448RB

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Description

ELISA

Human TfR/Transferrin R/CD71 quantitates human TfR/Transferrin R/CD71 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human TfR/Transferrin R/CD71.

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number 2610028K12Rik; AI195355; AI426448; AU015758; CD antigen CD71; CD71; E430033M20Rik; I79
Assay Range 0.819 to 200 ng/mL
Assay Sensitivity 0.81 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 7037
Gene Symbol TFRC
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Transferrin Receptor (CD71)
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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