Proteintech™ Human TDP-43 ELISA Kit

Produktkode 16851436

missing translation for 'mfr': Proteintech™ KE0000596T

Beskrivelse

KE00005 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA) and t is to be used to detect and quantify protein levels of endogenous TDP-43

• TARDBP was firstly found as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.
• It was reported to regulate alternate splicing of the CFTR gene. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP43, is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in Amyotrophic lateral sclerosis (ALS).
• KE00005 is used to quantify TDP-43 level in vivo and it is suitable for sample of serum or plasma.
• KE00009 is used to quantify TDP-43 level in vivo and it is suitable for sample of CSF and cell lysates.
• The assay recognizes human TDP-43. An antibody specific for TDP-43 has been pre-coated onto the microwells.
• The TDP-43 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for TDP-43 is added to detect the captured TDP-43 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent.
• Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Tekniske data

• Accession Number: NP_031401
• Assay Range: 78.1 to 5000 pg/mL
• Assay Sensitivity: 5 pg/mL
• Certifications/Compliance: ISO 13485
• Detection Method: Sandwich ELISA, Double Antibody
• Immunoassay Kit Format: Coated ELISA Kit
• Sample Type: Plasma, Serum
• Substrate: DAB
• Target Species: Human
• Gene ID (Entrez): 23435
• Host Species: Human
• Immunoassay Kit Grade: Solid
• Interassay CV: <10%
• Intraassay CV: <10%
• Shipping Condition: Blue ice
• Storage Requirements: -20°C to +4°C
• Total Assay Time: 4.5 hr.
• UniProt ID: Q13148

Sikkerhed og håndtering

Produktidentifikator

• 96TEST Human TDP-43 ELISA Kit

Signalord

• Warning

Farekategori

• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Faresætning

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.

Sikkerhedssætning

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P234-Keep only in original container.
• P264-Wash skin thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P330-Rinse mouth.
• P362-Take off contaminated clothing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplerende oplysninger

• MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine