Invitrogen™ Human TNFR1 (Soluble-60 kDa) ELISA Kit

Product Code. 15541527

Brand: Invitrogen™ BMS203

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal polyclonal antibody to human sTNF-R (60 kDa)
HRP-Conjugate anti-human sTNF-R (60 kDa) monoclonal antibody
Human sTNF-R (60 kDa) Standard, 10ng/mL upon dilution
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

ELISA

The Human Soluble Tumor Necrosis Factor Receptor 1 (sTNFR1-60 kDa) ELISA quantitates sTNFR1 in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu sTNFR1. Principle of the method The Human sTNFR1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.

Specifications

• Accession Number: P19438
• Assay Range: 0.08 to 5.0 ng/mL
• Assay Sensitivity: 0.05 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant, Urine
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 7132
• Gene Symbol: CD120a,FPF,TBP1,TNF-R,TNF-R-I,TNF-R55,TNFAR,TNFR1,TNFR55,TNFR60,p55,p55-R,p60
• Interassay CV: 8.6%
• Intraassay CV: 1.9
• Kit Contents: Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, Detection Antibody Conjugate: anti-rabbit-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 10 μL, Urine 10 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Tumor necrosis factor receptor 1, TNF-R1, CD120a
• Target: TNFR1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 10 mins