Invitrogen™ Human STK25 (aa 398-426) Control Fragment Recombinant Protein

Product Code. 30196785

Brand: Invitrogen™ RP100530

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (97%), Rat (97%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61445 (PA5-61445. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

STK25 (YSK1) is a serine/threonine kinase that belongs to the GCK family of kinases. It has been implicated as an upstream regulator of MAP kinase signaling pathways. Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Specifications

• Accession Number: O00506
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 10494
• Name: Human STK25 (aa 398-426) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 1500019J11Rik; AU018434; C86992; hypothetical protein; serine/threonine kinase 25; serine/threonine kinase 25 (STE20 homolog, yeast); serine/threonine kinase 25 (Ste20, yeast homolog); serine/threonine kinase 25 (yeast); serine/threonine-protein kinase 25; SOK1; SOK-1; Ste20, yeast homolog; ste20/oxidant stress response kinase 1; Ste20-like; ste20-like kinase; sterile 20/oxidant stress-response kinase 1; STK25; STK25 protein; Yeast Sps1/Ste20-related kinase 1; Ysk1
• Common Name: STK25
• Gene Symbol: STK25
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: PGISDKLMVHLVERVQRFSHNRNHLTSTR
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining