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Invitrogen™ PSGL-1/CD162 (Soluble) Human ELISA Kit

For the quantitative detection of human PSGL-1.

£451.00 - £3070.00

Specifications

Accession Number Q14242
Assay Range 1.56-50 U/mL
Assay Sensitivity 1.0 U/mL
Conjugate Biotin
Product Type ELISA Kit
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human PSGL-1
Biotin-Conjugate anti-human PSGL-1 monoclonal antibody
Streptavidin-HRP
Human PSGL-1 Standard lyophilized, 100 U/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

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Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15521837
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Invitrogen™
BMS255
96 Tests
£451.00
96 tests
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15531837
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Invitrogen™
BMS255TEN
10 x 96 Tests
£3070.00
Pack of 10
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Estimated Shipment: 11-04-2024
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Description

Description

The Human P-selectin Glycoprotein Ligand-1 (Hu PSGL-1/CD162) ELISA quantitates Hu PSGL-1/CD162 in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PSGL-1/CD162. Principle of the method The Human PSGL-1/CD162 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
Specifications

Specifications

Q14242
1.0 U/mL
ELISA Kit
Human
Colorimetric Microplate Reader
CD162,CLA,PSGL-1,PSGL1
3.2%
HRP
Serum, 15 μL; Supernatant, 100 μL
P-selectin glycoprotein ligand 1, CD162 antigen
1 hr. 20 min.
1.56-50 U/mL
Biotin
Serum, Supernatant
ELISA
6404
6.6%
Pre-coated 96 well plate, Adhesive Plate Covers, Sample Diluent, Sample Diluent, Assay Buffer concentrate, SAV-HRP, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls
RUO
2°C to 8°C
Human
3 hr. 10 min.
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