ACROBiosystems Human Sonic Hedgehog / Shh Protein, His Tag

Product Code.	Quantity	unitSize
15807687	1 mg	1mg
15817687	50 μg	50µg

Product Code. 15807687 Supplier ACROBiosystems Supplier No. SH7H52291MG

Description

Sonic hedgehog protein (SHH) is also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specifications

• Accession Number: NP_000184
• Molecular Weight (g/mol): 20.4 kDa
• Quantity: 1 mg
• Source: Human Sonic Hedgehog, His Tag (SH7-H5229) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184).
• Immunogen: Cys 24 - Gly 197
• Storage Requirements: -20°C
• Gene Alias: SHH,HHG-1,Sonic hedgehog,HLP3,HPE3,SMMCI,TPT,TPTPS
• Common Name: Sonic Hedgehog
• Conjugate: Unconjugated
• Species: Human
• Protein Tag: His Tag
• Buffer: PBS, pH7.4
• Endotoxin Level: Less than 1.0 EU / μg by the LAL method.
• Expression System: HEK293
• Form: Powder
• Protein Subtype: Cytokines and Growth Factors
• Purity or Quality Grade: >95% as determined by SDS-PAGE