Invitrogen™ Human ICAM-1 ELISA Kit, Extra Sensitive

Product Code. 15501817

Brand: Invitrogen™ BMS241

Beschreibung

Beinhaltet

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-1
HRP-Conjugate anti-human sICAM-1 monoclonal antibody
Human sICAM-1 Standard, 10ng/mL
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

ELISA

The Human Intercellular Adhesion Molecule-1 (Hu ICAM-1) ELISA quantitates Hu ICAM-1 in human serum, plasma, amniotic fluid, spontaneous urine. bile, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ICAM-1. Principle of the method The Human ICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

Spezifikation

• Accession Number: P05362
• Assay Range: 0.63 to 10.0 ng/mL
• Assay Sensitivity: 0.33 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Amniotic Fluid, Bile, Plasma, Serum, Supernatant, Urine
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3383
• Gene Symbol: BB2,CD54,P3.58
• Interassay CV: 7.8%
• Intraassay CV: 5.6
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Amniotic Fluid 100 μL, Bile 100 μL, Plasma 10 μL, Serum 10 μL, Supernatant 100 μL, Urine 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Intercellular adhesion molecule 1, CD54 antigen
• Target: ICAM-1 (CD54)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 10 mins