Invitrogen™ Human SF3B1 (aa 33-117) Control Fragment Recombinant Protein

Product Code. 30205002

Brand: Invitrogen™ RP101202

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-83997 (PA5-83997. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex. The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

Specifications

• Accession Number: O75533
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 23451
• Name: Human SF3B1 (aa 33-117) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 155 kDa; 2810001M05Rik; AA409119; Hsh155; LOW QUALITY PROTEIN: splicing factor 3 B subunit 1; MDS; pre-mRNA processing 10; pre-mRNA splicing factor SF3b, 155 kDa subunit; pre-mRNA-splicing factor SF3b 155 kDa subunit; PRP10; PRPF10; SAP 155; SAP155; SF3B1; SF3b155; spliceosome-associated protein 155; Splicing factor 3 B subunit 1; splicing factor 3 b subunit 1, 155 kDa; splicing factor 3 b, subunit 1; splicing factor 3 b, subunit 1, 155 kDa; splicing factor 3 b, subunit 1, 155 kD; splicing factor 3 b, subunit 1, 155 kDa; TA-8; Targ4; transforming growth factor alpha regulated gene 4; tst; wu:fb99f09
• Common Name: SF3B1
• Gene Symbol: SF3B1
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: LDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADRED
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining