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Invitrogen™ E-selectin (Soluble) Human ELISA Kit

For the quantitative detection of human sE-selectin.

£437.00 - £2790.00

Specifications

Accession Number P16581
Assay Range 1.6-50 ng/mL
Assay Sensitivity 0.3 ng/mL
Conjugate HRP
Product Type ELISA Kit
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sE-selectin
HRP-Conjugate anti-human sE-selectin monoclonal antibody
Human sE-selectin Standard lyophilized, 100ng/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

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Description

Description

The Human e-Selectin/Endothelial Leukocyte Adhesion Molecule-1 (Hu ELAM-1) ELISA quantitates Hu ELAM-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ELAM-1. Principle of the method The Human ELAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
Specifications

Specifications

P16581
0.3 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
CD62E,ELAM,ELAM1,ESEL,LECAM2
5.4%
HRP
Plasma, 20 μL; Serum, 20 μL; Supernatant, 20 μL
ELAM1, endothelial adhesion molecule 1, CD62E
1 hr. 20 min.
1.6-50 ng/mL
HRP
Plasma, Serum, Supernatant
ELISA
6401
6%
Pre-coated 96 well plate, Adhesive Plate Covers, Standard, Assay Buffer concentrate, Assay Buffer concentrate, SAV-HRP, SAV-HRP, Wash Buffer, Stop Solution, Controls, Controls
RUO
2°C to 8°C
Human
2 hr. 10 min.
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Product Certifications

For Research Use Only