Invitrogen™ E-selectin (Soluble) Human ELISA Kit

Product Code. 15521577

Brand: Invitrogen™ BMS205TEN

Description

Includes

• Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sE-selectin
• HRP-Conjugate anti-human sE-selectin monoclonal antibody
• Human sE-selectin Standard lyophilized, 100ng/mL upon reconstitution
• Control high, lyophilized
• Control low, lyophilized
• Sample Diluent
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
• Substrate Solution (tetramethyl-benzidine)
• Stop Solution (1M Phosphoric acid)
• Blue-Dye
• Green-Dye
• Adhesive Films

For the quantitative detection of human sE-selectin.

The Human e-Selectin/Endothelial Leukocyte Adhesion Molecule-1 (Hu ELAM-1) ELISA quantitates Hu ELAM-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ELAM-1. Principle of the method The Human ELAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

E-selectin (CD62E, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. E-selectin is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. E-selectin is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. E-selectin binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human E-selectin and to be useful for flow cytometry. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Specifications

• Accession Number: P16581
• Assay Range: 1.6-50 ng/mL
• Assay Sensitivity: 0.3 ng/mL
• Conjugate: HRP
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6401
• Gene Symbol: CD62E,ELAM,ELAM1,ESEL,LECAM2
• Interassay CV: 6%
• Intraassay CV: 5.4%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma, 20 μL; Serum, 20 μL; Supernatant, 20 μL
• Storage Requirements: 2°C to 8°C
• Synonym: ELAM1, endothelial adhesion molecule 1, CD62E
• Target: Human
• Test Time: 1 hr. 20 min.
• Total Assay Time: 2 hr. 10 min.

For Research Use Only