Invitrogen™ APO-1/FAS (Soluble) Human ELISA Kit

For the quantitative detection of human sAPO-1/Fas.

Brand:  Invitrogen™ BMS245TEN

Product Code. 15521827

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sAPO-1/Fas
Biotin-Conjugate anti-human sAPO-1/Fas monoclonal antibody
Streptavidin-HRP
Human sAPO-1/Fas Standard lyophilized, 2ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

The Human Soluble APO-1/Fas ELISA quantitates Hu sAPO-1/Fas in human serum, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu sAPO-1/Fas. Principle of the method The Human APO-1/Fas solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

Specifications

• Accession Number: P25445
• Assay Sensitivity: 13.2 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: ALPS1A,APO-1,APT1,CD951,FASTM,TNFRSF6,FAS
• Intraassay CV: 4.5%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 2 hr. 10 min.

• Assay Range: 15.6-1,000 pg/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 355
• Interassay CV: 3.1%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard, Assay Buffer concentrate, Assay Buffer concentrate, Biotinylated Detection Antibody, Wash Buffer, Wash Buffer, Stop Solution, Stop Solution, Controls
• Quantity: 10 x 96 Tests
• Sample Volume: Serum, 10 μL; Supernatant, 10 μL
• Synonym: CD95, Fas receptor, APT 1, ALPS 1 A, TNFRSF6
• Test Time: 1 hr. 20 min.

For Research Use Only