Invitrogen™ Human RNASE3 ELISA Kit

Product Code. 30245384

Brand: Invitrogen™ EEL208

Description

ELISA

ECP (eosinophil cationic protein), also known as RNS3 or RNASE3 (ribonuclease, RNase A family, 3), is a 160 amino acid potent cytotoxic secretory protein that belongs to the pancreatic ribonuclease family. ECP localizes to the granule matrix of the eosinophil and is involved in the immune response system. ECP is a cytotoxin, neurotoxin and helminthotoxin that is secreted by activated human eosinophils. ECP exhibits anti-parasitic, antibacterial and ribonucleolytic activities. It has been suggested that ECP induces the neurotoxic effect known as the Gordon phenomenon, a syndrome manifested by ataxia, muscular rigidity, paralysis and tremor that may lead to death. ECP is considered a marker of eosinophilic inflammation and high levels have been found in cases of active asthma and other allergic diseases.

Specifications

• Accession Number: P12724
• Assay Range: 39.06 to 2500 pg/mL
• Assay Sensitivity: 23.44 pg/mL
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: ELISA Microplate (Dismountable)
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6037
• Gene Symbol: RNASE3
• Interassay CV: <10
• Intraassay CV: <10
• Kit Contents: Micro ELISA Plate (Dismountable), Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma: 100 μL, Serum: 100 μL
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: RNASE3
• Test Time: 1 hr. 20 min.
• Total Assay Time: 3 hr. 30 min.