Invitrogen™ Human RANTES ELISA Kit

Product Code. 10763115

Brand: Invitrogen™ EHRNTS5

Description

ELISA

The Human RANTES ELISA quantitates Hu RANTES in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu RANTES. Principle of the method The Human RANTES solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

Specifications

• Accession Number: P13501
• Assay Range: 51.2 to 2000 pg/mL
• Assay Sensitivity: 2 pg/mL
• Description: Human RANTES ELISA Kit
• Conjugate: HRP
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant, Urine
• Specificity: No significant cross-reaction with human Eotaxin, GROa, GROß, MIP-1 A, MIP-1ß, MCP-1, MCP-2, MCP-3, MCP-4, G-CSF, GM-CSF, IFNa, IFNg, IL-1 A, IL-1ß, IL-1 RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, TNFa, TNFß, or rat RANTES.
• Sufficient For: 5 x 96 microplate wells
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6352
• Gene Symbol: D17S136E,RANTES,SCYA5,SIS-delta,SISd,TCP228,eoCP
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Measurement: Absorbance at 450 nm minus absorbance (at 550 nm)
• Precision: Intra-assay CV <10%; inter-assay CV <10%
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 1 μL, Serum 1 μL, Supernatant 50 μL, Urine 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CCL5 (RANTES)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs