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Invitrogen™ Human QTRT1 (aa 212-300) Control Fragment Recombinant Protein

Product Code. 30182539
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Quantity:
100 μL
Unit Size:
100µL
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Product Code. 30182539

Brand: Invitrogen™ RP99237

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This item is not returnable. View return policy

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (94%), Rat (94%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61795 (PA5-61795. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD subunit (USP14; MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001 [PubMed 11255023]).
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q9BXR0
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 81890
Name Human QTRT1 (aa 212-300) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias 2610028E17Rik; FP3235; Guanine insertion enzyme; QTRT1; queuine tRNA-ribosyltransferase; queuine tRNA-ribosyltransferase 1; queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase); queuine tRNA-ribosyltransferase catalytic subunit 1; queuine tRNA-ribosyltransferase; queuine tRNA-ribosyltransferase catalytic subunit 1; similar to queuine tRNA-ribosyltransferase 1; Tgt; TGT, 43-KD subunit; TGT, catalytic subunit; TGUT; tRNA-guanine transglycosylase; zgc:66378
Common Name QTRT1
Gene Symbol QTRT1
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence TCLEEMTKRDVPGFAIGGLSGGESKSQFWRMVALSTSRLPKDKPRYLMGVGYATDLVVCVALGCDMFDCVFPTRTARFGSALVPTGNLQ
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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