Invitrogen™ Human PSME1 (aa 151-249) Control Fragment Recombinant Protein

Product Code. 30197105

Brand: Invitrogen™ RP89237

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (96%), Rat (96%). This recombinant protein control fragment may be used for blocking experiments. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (approximately 31), barrel-shaped molecular machine with an apparent molecular weight of approximately 2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

Specifications

• Accession Number: Q06323
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 5720
• Name: Human PSME1 (aa 151-249) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 11 S regulator complex subunit alpha; 29-kD MCP activator subunit; activator of multicatalytic protease subunit 1; AW413925; epididymis secretory sperm binding protein Li 129 m; HEL-S-129 m; I79_008549; IFI5111; IGUP I-5111; interferon gamma up-regulated I-5111 protein; interferon-gamma IEF SSP 5111; interferon-gamma-inducible protein 5111; MGC8628; OTTHUMP00000164702; PA28 alpha subunit; PA28a; PA28alpha; protease (prosome, macropain) 28 subunit, alpha; proteasome (prosome, macropain) 28 subunit, alpha; proteasome (prosome, macropain) activator subunit 1; proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); proteasome activator 28 subunit alpha; Proteasome activator complex subunit 1; proteasome activator subunit 1; Psme1; REGalpha; REG-alpha
• Common Name: PSME1
• Gene Symbol: PSME1
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: AVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining