Invitrogen™ Human Profilin 1 ELISA Kit

Product Code.	Quantity
30827736	96 Tests

Product Code. 30827736 Supplier Invitrogen™ Supplier No. EEL187

Description

ELISA

The Human Profilin 1 (PFN1) ELISA quantitates PFN1 in serum, plasma and other biological fluids. Principle of the method The Human Profilin 1 (PFN1) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme antibody target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Specifications

• Accession Number: P07737
• Assay Range: 78.13-5000 pg/mL
• Assay Sensitivity: 46.88 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: actin binding protein; ALS18; Epididymis tissue protein Li 184a; Pfn; Pfn1; profilin 1; profilin I; Profilin-1actin binding protein; ALS18; Epididymis tissue protein Li 184a; Pfn; Pfn1; profilin 1; profilin I; Profilin-1;
• Gene ID (Entrez): 5216
• Gene Symbol: Pfn1
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Profilin 1
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min