Invitrogen™ Human PPP1R1A (aa 105-171) Control Fragment Recombinant Protein

Product Code. 30213245

Brand: Invitrogen™ RP102910

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (57%), Rat (57%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-59618 (PA5-59618. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Protein phosphatase inhibitor-1 (also designated inhibitor of protein phospha-tase 1, IPP-1 and I-1) plays a role in regulating the phosphorylation of other proteins, and is itself phosphorylated by a cyclic AMP-dependent protein kinase at Threonine 35. In addition, the proline-directed kinases Cdk1, Cdk5, and mitogen-activated protein kinase (MAPK) mediate in vitro phosphorylation of IPP-1 at the phylogenetically conserved position Serine 67. In striatal tissues, glutamate-dependent regulation of N-methyl-d-aspartic acid-type channels influences IPP-1 phosphorylation at Ser 67. The localization and expression of IPP-1 suggests that it may play discrete roles in certain regions and developing stages of the brain, independent of the regulation of protein phosphatase type 1 (PP-1). PP-1 binds to both phosphorylated and dephosphorylated IPP-1. Conversion of PP-1 to a Mn++-dependent state appears to play a role in its regulation by IPP-1. IPP-1 attenuates the activity of glycogen phosphorylase and is thought to be important in the hormonal control of glycogen metabolism.

Specifications

• Accession Number: Q13522
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 5502
• Name: Human PPP1R1A (aa 105-171) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 0610038N18Rik; I1; I-1; inhibitor-1; Ipp1; IPP-1; PP1 inhibitor 1; Ppp1r1a; protein phosphatase 1 regulatory inhibitor subunit 1 A; protein phosphatase 1 regulatory subunit 1 A; protein phosphatase 1, regulatory (inhibitor) subunit 1 A; protein phosphatase inhibitor 1; protein phosphatase inhibitor-1
• Common Name: PPP1R1A
• Gene Symbol: Ppp1r1a
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: AESTETQESRPPGIPDTEVESRLGTSGTAKKTAECIPKTHERGSKEPSTKEPSTHIPPLDSKGANSV
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining