Invitrogen™ Human POLR2K (aa 1-25) Control Fragment Recombinant Protein

Product Code. 30210963

Brand: Invitrogen™ RP108859

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (96%), Rat (96%). This recombinant protein control fragment may be used for blocking experiments. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. POLR2K (polymerase (RNA) II (DNA directed) polypeptide K), also known as RPB12, RPABC4, RPB7.0, hRPB7.0, hsRPB10a, RPB10alpha or ABC10-alpha, is a 58 amino acid nuclear protein belonging to the archaeal rpoP/eukaryotic RPC10 RNA polymerase subunit family. The gene encoding POLR2K is located on human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies.

Specifications

• Accession Number: P53803
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 5440
• Name: Human POLR2K (aa 1-25) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: ABC10-alpha; DNA directed RNA polymerases I, II, and III 7.0 kda polypeptide; DNA-directed RNA polymerase II subunit K; DNA-directed RNA polymerases I, II, and III subunit RPABC4; hRPB7.0; hsRPB10a; MafY; mCG145114-like; metallothionein-I gene transcription activator; Mt1a; POLR2K; polymerase (RNA) II (DNA directed) polypeptide K; polymerase (RNA) II (DNA directed) polypeptide K, 7.0 kDa; polymerase (RNA) II subunit K; RNA polymerase II 7.0 kDa subunit; RNA polymerase II subunit K; RNA polymerases I, II, and III subunit ABC4; RPABC4; RPB10alpha; RPB12; RPB7.0
• Common Name: POLR2K
• Gene Symbol: POLR2K
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: MDTQKDVQPPKQQPMIYICGECHTE
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining