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Invitrogen™ Human POLK (aa 406-480) Control Fragment Recombinant Protein

Product Code. 30196376
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Quantity:
100 μL
Unit Size:
100µL
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Product Code. 30196376

Brand: Invitrogen™ RP107689

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This item is not returnable. View return policy

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (85%), Rat (85%). This recombinant protein control fragment may be used for blocking experiments. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q9UBT6
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 51426
Name Human POLK (aa 406-480) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias DinB homolog 1; DINB protein; Dinb1; DINP; DNA damage-inducible protein b; DNA polymerase kappa; DNA-directed DNA polymerase kappa; LOW QUALITY PROTEIN: DNA polymerase kappa; POLK; POLQ; polymerase (DNA directed) kappa; polymerase (DNA directed), kappa; polymerase (DNA) kappa
Common Name POLK
Gene Symbol POLK
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence HLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKGRTVTIKLKNVNFEVKTRASTVSSVVST
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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