Invitrogen™ Human PGAM1 (aa 26-50) Control Fragment Recombinant Protein

Product Code. 30209755

Brand: Invitrogen™ RP105153

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-84457 (PA5-84457. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

PGAM1 belongs to the phosphoglycerate mutase family. PGAM1 is an important component of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM1 protein is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in PGAM1 cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specifications

• Accession Number: P18669
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 5223
• Name: Human PGAM1 (aa 26-50) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 2310050F24Rik; BPG-dependent PGAM 1; CDABP0006; epididymis secretory protein Li 35; HEL-S-35; PGAM1; Pgam-1; PGAMA; PGAM-B; Pgmut; Phosphoglycerate mutase 1; phosphoglycerate mutase 1 (brain); phosphoglycerate mutase A, nonmuscle form; phosphoglycerate mutase isozyme B; RP11-452K12.8; similar to phosphoglycerate mutase
• Common Name: PGAM1
• Gene Symbol: PGAM1
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: YDADLSPAGHEEAKRGGQALRDAGY
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining