Invitrogen™ PEI-Transferrinfection Kit

Product Code. 15541307

Brand: Invitrogen™ BMS1003a

Description

Includes

• Transferrin-Polyethylenimine-conjugate, lyophilized, 1 mg/mL upon reconstitution; iron incorporated
• Deferrioxamine, lyophilied, 50 mM upon reconstitution
• HBS buffer concentrate (20x)
• (400mM Hepes, pH 7.3, 3M NaCl)

Nucleic acid delivery system based on transferrin receptor-mediated endocytosis to carry DNA into cells and the gene transfer efficiency of PEI/DNA complexes

• Method results in a 30 to 1000-fold enhanced transfection efficiency depending on the cell line
• An extremely gentle DNA transfection method which employs physiological uptake mechanisms of the cell
• Transfection efficacy depends on the cell type, the level of surface transferrin receptor expression
• Very high transfection rates have been shown for the tested tumor cell lines B16F10 melanoma, Neuro 2A neuroblastoma and a variety of primary human melanoma cell lines
• In other established cell lines, such as HeLa, CHO, Jurkat, K562, HepG2 and COS the PEI-Transferrinfection works with high efficiences, excellent reproducibility and with the advantage of being an extremely gentle procedure

Specifications

• Classification: Chemically Defined
• Cell Type: Tumor Cell Lines, Established Cell Lines
• Format: Kit
• Sample Type: Plasmid DNA
• Transfection Technique: Transferrin Receptor-mediated Endocytosis
• For Use With (Application): Transfection
• Product Type: Transferrinfection Kit
• Quantity: 16 Transferrinfections