Gibco™ Human PDGF-CC Recombinant Protein, PeproTech®

Product Code. 17890283

Brand: Gibco™ 10000CC100UG

Description

Recombinant Protein

100-00CC-1MG will be provided as 4 x 250 μg (100-00CC-250UG). 100-00CC-500UG will be provided as 2 x 250 μg (100-00CC-250UG). Recombinant Human PDGF-CC is a 25.0 kDa protein consisting of two identical disulfide-linked, 112 amino acid, polypeptide chains. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel, disulfide-linked dimer of two 12-14 kDa, polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-alpha and PDGFR-beta, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules' epithelial cell development. Mature PDGFs are stored in platelet alpha-granules, and are released upon platelet activation. PDGF-AA, -AB, -BB and -CC signal primarily through the PDGFR-alpha receptor, whereas PDGF-DD interacts almost exclusively with the PDGFR-beta receptor.

Specifications

• Accession Number: Q9NRA1
• For Use With (Application): Control, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 56034
• Molecular Weight (g/mol): 25 kDa
• Name: Human PDGF-CC
• Purification Method: Purified
• Quantity: 100 μg
• Source: E. coli
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: PDGF-C; Platelet Derived Growth Factor C; SCDGF; Spinal Cord-Derived Growth Factor
• Common Name: PDGF-CC
• Gene Symbol: PDGFC
• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected The ED50 for this effect is 15-20 ng/ml
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MVVDLNLLTE EVRLYSCTPR NFSVSIREEL KRTDTIFWPG CLLVKRCGGN CACCLHNCNE CQCVPSKVTK KYHEVLQLRP KTGVRGLHKS LTDVALEHHE ECDCVCRGST GG
• Content And Storage: -20°C
• Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected The ED50 for this effect is 15-20 ng/ml.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.