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Invitrogen™ PDGF-BB (CSRP2) Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHCSRP2

362.68 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.00400kg

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Product Code. 15454953

  • £544.00 / 1 set
Estimated Shipment: 22-04-2024
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Description

Description

The Human Platelet-Derived Growth Factor (CSRP2) (Hu PDGF-BB) ELISA quantitates Hu PDGF-BB in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu PDGF-BB. Principle of the method The Human PDGF-BB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor alpha polypeptide , where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Specifications

Specifications

P01127
1 pg/mL
Solid-phase sandwich ELISA
ELISA Kit
Cell Lysates
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
5155
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Differentiation
2°C to 8°C
Human
4 hr. 45 min.
0.549-400 pg/mL
1pg/mL to 400pg/mL
Biotin
Other Proteins
The antibody pair provided in this kit recognizes human PDGF-BB.
Human
Colorimetric Microplate Reader
IBGC5,PDGF-2,PDGF2,SIS,SSV,c-sis
<10%
HRP
RUO
Cell Lysate,20 μL
CRP2, LMO5, SmLIM
1 hr. 20 min.
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