Invitrogen™ Human PD-1 His-tag Recombinant Protein

Product Code.	Quantity	unitSize
17164203	50 μg	50µg
17154203	5 x 50 μg	Pack of 5
17144203	5 x 5 μg	Pack of 5

Product Code. 17164203 Supplier Invitrogen™ Supplier No. A42533

Description

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of 'danger signals' such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Specifications

• Accession Number: Q15116
• For Use With (Application): Control, Binding Assay, Immunohistochemistry, Surface Plasmon Resonance, Western Blot
• Formulation: 50mM MOPS with 0.01% Tween 80, 5% mannitol, 500mM NaCl, 5% trehalose and no preservative; pH 7
• Gene ID (Entrez): 5133
• Molecular Weight (g/mol): 17.4 kDa
• Name: Human PD-1 His-tag
• pH Range: 7
• Purification Method: Purified
• Quantity: 50 μg
• Source: HEK293 cells
• Storage Requirements: -20°C or -80°C if preferred
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
• Common Name: PD-1 (CD279)
• Gene Symbol: Pdcd1
• Biological Activity: 1. Immobilized Human PD-1 His-tag Recombinant Protein at 2 μg/mL (100 μL/well) can bind PD-L1 Protein, Human, Recombinant (ECD, hFc Tag), the EC50 of human B7-H1 is 150-600 ng/mL 2. Immobilized Human PD-1 His-tag Recombinant Protein (at 2 μg/mL (100 μL/well) can bind Anti-PD1 (MDX)-IgG4 Antibody (Nivolumab), the EC50 is 8-60 ng/mL 3. Loaded Anti-human PD1 antibody, IgG4 on ProA Biosensor, can bind Human PD-1 His-tag Recombinant Protein with an affinity constant of 2.44 nM as determined in a BLI assay (Sartorius Octet RED384) (Routinely tested). 4.Captured Anti-PD1 Mab (Human IgG4) on proA Chip can bind Human PD-1 His-tag Recombinant Protein with an affinity constant of 10.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).)
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Human PD1, amino acids Met1-Gln167 (Accession # NP_005009.2) with a C-terminal His-tag
• Content And Storage: Includes 1 vial of lyophilized protein. Store at -20 °C.
• Activity: 1. Immobilized Human PD-1 His-tag Recombinant Protein at 2 μg/mL (100 μL/well) can bind PD-L1 Protein, Human, Recombinant (ECD, hFc Tag), the EC50 of human B7-H1 is 150-600 ng/mL.<br><br>2. Immobilized Human PD-1 His-tag Recombinant Protein at 2 μg/mL (100 μL/well) can bind Anti-PD1 (MDX)-IgG4 Antibody (Nivolumab), the EC50 is 8-60 ng/mL.<br><br>3. Loaded Anti-human PD1 antibody, IgG4 on ProA Biosensor, can bind Human PD-1 His-tag Recombinant Protein with an affinity constant of 2.44 nM as determined in a BLI assay (Sartorius Octet RED384) (Routinely tested).<br><br>4.Captured Anti-PD1 Mab (Human IgG4) on proA Chip can bind Human PD-1 His-tag Recombinant Protein with an affinity constant of 10.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Endotoxin Level: < 1 EU/μg
• Expression System: HEK293 cells
• Protein Family: CD Proteins
• Form: Lyophilized
• Protein Subtype: Other Proteins
• Research Category: Apoptosis, Immunology, Inflammation
• Purity or Quality Grade: >95 % as determined by SDS-PAGE. >90 % as determined by SEC-HPLC.
• Protein: programmed cell death 1