Proteintech™ Human P21 ELISA Kit

Product Code. 16851466

Brand: Proteintech™ KE0004996T

Description

Solid phase sandwich ELISA for the quantitative determination of P21 in samples of human and mouse origin

• CDKN1A (p21, CIP1, WAF1) a cyclin-dependent kinase inhibitor is necessary for proper control of cell cycle and premature senescence
  • p21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at G1/s and G2/M transitions
    • Mediator of p53 to induce cell cycle arrest in G1
    • Can interact with proliferating cell nuclear antigen, plays regulatory role in S phase DNA replication and DNA damage repair
    • Overexpression decreases expression levels of cell cycle progression genes and upregulation senescence-inducing genes

Process:

• Antibody specific for P21 pre-coated onto microwells
• P21 protein in samples captured by the coated antibody after incubation
• After extensive washing, another antibody specific for P21 is added to detect captured P21 protein
• For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
• Solution containing sulfuric acid used to stop color development and color intensity
  • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm

Specifications

• Accession Number: NP_000380
• Assay Range: 0.25 to 16 ng/mL
• Assay Sensitivity: 0.1 ng/mL
• Certifications/Compliance: ISO 13485
• Detection Method: Sandwich ELISA, Double Antibody
• Immunoassay Kit Format: Coated ELISA Kit
• Sample Type: Plasma, Serum
• Substrate: DAB
• Target Species: Human
• Gene ID (Entrez): 1026
• Host Species: Human
• Immunoassay Kit Grade: Solid
• Interassay CV: <10%
• Intraassay CV: <10%
• Shipping Condition: Blue ice
• Storage Requirements: -20°C to +4°C
• Total Assay Time: 3.5 hr.
• UniProt ID: P38936

Safety and Handling

Product Identifier

• 96TEST Human P21 ELISA Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P234-Keep only in original container.
• P264-Wash skin thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P330-Rinse mouth.
• P362-Take off contaminated clothing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine