Gibco™ Human OATP1B3 (G) SLC Transporter Cells

Product Code.	Quantity	unitSize
17116632	8–10M cells	Each

Product Code. 17116632 Supplier Gibco™ Supplier No. GM1106G

Description

HEK293 cells that transiently overexpress the OATP1B3 solute carrier (SLC) transport protein

• TRANSiPORT Human OATP1B3 (G) SLC Transporter Cells are HEK293 cells that transiently overexpress the OATP1B3 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OATP1B3 SLC transporter protein is found in sinusoidal hepatocytes.
• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio
• Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.
• Mock HEK293 cells (Cat. No. GM1101G) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.
• The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Specifications

• Content And Storage: Store in liquid nitrogen vapor phase.
• Cell Line: 293 (HEK)
• Culture Type: Mammalian Cell Culture
• Species: Human
• No. of Cells: 8-10 x 10⁶
• Product Type: SLC Transporter Cell
• Quantity: 8–10M cells

Frequently Asked Questions (FAQs)

What are TRANSiPORT human solute carrier (SLC) transporter cells?

TRANSiPORT human SLC transporter cells are HEK293 cells that transiently overexpress a solute carrier (SLC) transporter protein. Solute carriers are one of two superfamilies of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes into and out of cells. Membrane transporters are found in hepatic cells, kidney proximal tubule cells, intestinal cells, and brain cells.

Is there a protocol available for the TRANSiPORT solute carrier (SLC) transporter cells?

Yes, the protocol is available here (https://www.genomembrane.com/information).

What should I use as a control with TRANSiPORT solute carrier (SLC) transporter cells?

Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of SLC transport due to the presence of endogenous transporters or non-specific binding.

With TRANSiPORT solute carrier (SLC) transporter cells, what should my choice of measurement be for SLC transporter activity?

Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

How many cells can I expect in one vial of TRANSiPORT solute carrier (SLC) transporter cells?

One vial contains 8-12 million cells with high viability, designed to seed one 24-well plate for single use.

What are the shipping and storage conditions for TRANSiPORT solute carrier (SLC) transporter cells?

Domestic shipments are on dry ice and shipped overnight. We ask our customers to store the product under liquid nitrogen after they receive it. International shipments are under liquid nitrogen conditions.