Gibco™ Human NOV Recombinant Protein, PeproTech®

Product Code. 17840873

Brand: Gibco™ 12026100UG

Description

Recombinant Protein

Recombinant Human NOV is a 36.2 kDa protein containing 331 amino acid residues. It is composed of four distinct structural domains (modules): the IGF binding protein (IGFBP) domain; the von Willebrand Factor C (VWFC) domain; the Thrombospondin type-I (TSP type-1) domain; and a C-terminal cysteine knot-like domain (CTCK). This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

NOV is a member of the CCN family of secreted, cysteine-rich regulatory proteins. The full-length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm's tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorigenicity and proliferation is correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and cell migration by signaling through specific cell-surface integrins, and by binding to heparin sulfate proteoglycans and to fibulin 1C. NOV has also been reported to exert proangiogenic activities.

Specifications

• Accession Number: P48745
• For Use With (Application): ELISA, Functional Assay, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 4856
• Molecular Weight (g/mol): 36.2 kDa
• Name: Human NOV
• Quantity: 100 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: C130088N23Rik; CCN family member 3; CCN3; Cellular communication network factor 3; IBP-9; IGF-binding protein 9; IGFBP9; IGFBP-9; Insulin-like growth factor-binding protein 9; Nephro blastoma-overexpressed gene protein homolog; nephroblastoma overexpressed; nephroblastoma overexpressed gene; nephroblastoma overexpressed gene protein homolog; nephroblastoma-overexpressed gene protein homolog; NOV; NovH; protein NOV homolog
• Common Name: NOV
• Gene Symbol: CCN3
• Biological Activity: Determined by a cell proliferation assay using BALB/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 ug/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.