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Invitrogen™ Human NOB1 (aa 211-312) Control Fragment Recombinant Protein

Product Code. 30180701
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Quantity:
100 μL
Unit Size:
100µL
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Product Code. 30180701

Brand: Invitrogen™ RP98724

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This item is not returnable. View return policy

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (92%), Rat (92%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61967 (PA5-61967. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q9ULX3
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 28987
Name Human NOB1 (aa 211-312) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias 1700021I09Rik; 20 S-pre-rRNA D-site endonuclease NOB1; adenocarcinoma antigen recognized by T lymphocytes 4; ART4; ART-4; MST158; MSTP158; nin one binding protein; NIN1/PSMD8 binding protein 1 homolog; NIN1/RPN12 binding protein 1 homolog; NIN1/RPN12 binding protein 1 homolog (S. cerevisiae); NIN1-binding protein; Nob1; Nob1p; phosphorylation regulatory protein HP-10; Pre-rRNA-processing endonuclease NOB1; Protein ART-4; PSMD8 binding protein 1; PSMD8BP1; RNA-binding protein NOB1; rRNA-binding endoribonuclease; YOR056C; YOR29-07
Common Name NOB1
Gene Symbol Nob1
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence PSNIKQIQQELEQCDVPEDVRVGCLTTDFAMQNVLLQMGLHVLAVNGMLIREARSYILRCHGCFKTTSDMSRVFCSHCGNKTLKKVSVTVSDDGTLHMHFSR
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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