Invitrogen™ Human NGAL ELISA Kit

Product Code. 11530271

Brand: Invitrogen™ KIT036

Description

ELISA

The Human NGAL (Hu Neutrophil Gelatinase-Associated Lipocalin) ELISA quantitates Hu NGAL in human serum, plasma, urine, tissue homogenates, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NGAL. Principle of the method The Human NGAL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.

Specifications

• Accession Number: P80188
• Assay Range: 10 to 1000 pg/mL
• Assay Sensitivity: 4 pg/mL
• Description: Human NGAL ELISA Kit
• Conjugate: HRP
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant, Tissue Homogenates, Urine
• Specificity: The two monoclonal antibodies against human NGAL used in the assay have been shown to bind to different preparations of recombinant human NGAL and to give a single band at 25 kDa on Western blot analysis of a reduced postnuclear supernatant from human neμt
• Sufficient For: 96 microplate wells
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3934
• Gene Symbol: 24p3,MSFI,NGAL,p25
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer Concentrate, Chromogen, Stop Solution, Controls
• Label or Dye: HRP
• Measurement: Absorbance at 450 nm minus absorbance (at 550 nm)
• Precision: Undetermined
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 10 μL, Tissue Homogenate 10 μL, Urine 10 μL
• Storage Requirements: 2°C to 8°C
• Target: NGAL
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 10 mins