Gibco™ Human Nesfatin-1 Recombinant Protein, PeproTech®

Product Code.	Quantity	unitSize
30132245	5 x 100 μg	Pack of 5
30130865	10 x 100 μg	Pack of 10
30131512	2 x 100 μg	Pack of 2

Product Code. 30132245 Supplier Gibco™ Supplier No. 30067500

Description

Recombinant Protein

300-67-1MG will be provided as 10 x 100 μg (300-67-100UG). 300-67-200UG will be provided as 2 x 100 μg (300-67-100UG). 300-67-500UG will be provided as 5 x 100 μg (300-67-100UG). Recombinant Human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.

Specifications

• Accession Number: P80303
• For Use With (Application): Functional Assay
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 4925
• Molecular Weight (g/mol): 9.7 kDa
• Name: Human Nesfatin-1
• Quantity: 5 x 100 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: AI607786; Calnuc; DNA-binding protein NEFA; epididymis secretory protein Li 109; Gastric cancer antigen Zg4; HEL-S-109; LOW QUALITY PROTEIN: nucleobindin-2; NEFA; nesfatin 1; nesfatin-1; Nucb2; nucleobindin 2; nucleobindin-2; Nucleobindin-2/Nesfatin-1; nucleobindin-2-like protein; nucleobinding 2; p54; prepronefastin; prepronesfatin
• Common Name: NUCB2
• Biological Activity: Determined by an in vivo assay using healthy wild type male mice (C57BL/6 J). Mice were treated via intraperitoneal injection once (at a dose of 4 ug Nesfatin-1/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.)
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL
• Content And Storage: -20°C
• Activity: Determined by an in vivo assay using healthy wild type male mice (C57BL/6 J). Mice were treated via intraperitoneal injection once (at a dose of 4 ug Nesfatin-1/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.)
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.