Invitrogen™ Human NEK8 (aa 375-449) Control Fragment Recombinant Protein

Product Code. 30181751

Brand: Invitrogen™ RP98306

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-111124 (PA5-111124. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

NEK8 serine/threonine kinase or NIMA-related kinase 8 is related to NIMA (never in mitosis, gene A) of Aspergillus nodulins and may play a role in cell cycle progression from G2 to M phase. NEK8 is part of a complex of ciliary proteins required for renal and cardiovascular development. NEK8 interacts with polycystin signal transduction pathways and may control the targeting of these ciliary proteins. Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia.Defects in NEK8 are the cause of nephronophthisis type 9 (NPHP9) [MIM:613824]. NPHP9 is an autosomal recessive disorder resulting in end-stage renal disease. It is a progressive tubulo-interstitial kidney disorder histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts.

Specifications

• Accession Number: Q86SG6
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 284086
• Name: Human NEK8 (aa 375-449) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 4632401F23Rik; b2b1449Clo; JCK; juvenile cystic kidney; NEK 8; NEK12A; Nek8; nephrocystin 9; never in mitosis A-related kinase 8; NIMA (never in mitosis gene a)- related kinase 8; NIMA (never in mitosis gene a)-related expressed kinase 8; NIMA related kinase 8; NIMA-family kinase NEK8; NIMA-related expressed kinase 8; NIMA-related kinase 12 A; NIMA-related kinase 8; nima-related protein kinase 12 A; NimA-related protein kinase 8; NPHP9; RHPD2; Serine/threonine-protein kinase Nek8; serine/thrionine-protein kinase NEK8
• Common Name: NEK8
• Gene Symbol: NEK8
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: QPQPQFISRFLEGQSGVTIKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHGSLTDISQPTIVEALLGYEMVQVA
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining