Invitrogen™ Human NEDD8 (aa 1-36) Control Fragment Recombinant Protein

Product Code. 30195257

Brand: Invitrogen™ RP108442

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%). This recombinant protein control fragment may be used for blocking experiments. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

Specifications

• Accession Number: Q15843
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 4738
• Name: Human NEDD8 (aa 1-36) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: nedd8; NEDD-8; NEDD8 ubiquitin like modifier; nedd8.L; nedd8.S; neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; neural precursor cell expressed, developmentally down-regulated 8 L homeolog; neural precursor cell expressed, developmentally down-regulated 8 S homeolog; neural precursor cell expressed, developmentally down-regulated gene 8; neural precursor cell-expressed developmentally down-regulated; Rub1; si:zc14a17.5; ubiquitin; ubiquitin-like protein Nedd8; XELAEV_18007009mg
• Common Name: NEDD8
• Gene Symbol: Nedd8
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGI
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining