Invitrogen™ Human Mylein Basic Protein ELISA Kit

Product Code. 30826228

Brand: Invitrogen™ EEL167

Description

ELISA

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise calledGolli-MBP) that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

Specifications

• Accession Number: P02686
• Assay Range: 15.63 to 1000 pg/mL
• Assay Sensitivity: 9.38 pg/mL
• Conjugate: Unlabeled
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4155
• Gene Symbol: MBP
• Interassay CV: <10
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: MBP
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 30 mins