Invitrogen™ Human MRPL47 (aa 194-250) Control Fragment Recombinant Protein

Product Code. 30193825

Brand: Invitrogen™ RP104838

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (65%), Rat (65%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-65479 (PA5-65479. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identified.

Specifications

• Accession Number: Q9HD33
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 57129
• Name: Human MRPL47 (aa 194-250) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 39 S ribosomal protein L47, mitochondrial; 4833424P18Rik; CGI-204; ENSMUSG00000051761; Gm9859; L47mt; metanephros-derived tubulogenic factor; Mitochondrial large ribosomal subunit protein uL29m; mitochondrial ribosomal protein L47; Mrpl47; MRP-L47; MTF/L47; nasopharyngeal carcinoma metastasis-related 1; nasopharyngeal carcinoma metastasis-related protein 1; NCM1
• Common Name: MRPL47
• Gene Symbol: MRPL47
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: YNRKRFFALPYVDHFLRLEREKRARIKARKENLERKKAKILLKKFPHLAEAQKSSLV
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining