Proteintech™ Human/Mouse SMN ELISA Kit

Product Code. 16831456

missing translation for 'mfr': Proteintech™ KE0002796T

Description

KE00027 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SMN ELISA kit is designed for the quantitative determination of SMN in samples of human and mouse origin

• An antibody specific for SMN has been pre-coated onto the microwells.
• The SMN protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SMN is added to detect the captured SMN protein.
• For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent.
• Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
• Survival Motor Neuron (SMN) protein required for efficient assembly of small nuclear ribonucleoprotein (snRNP) complexes is encoded by nearly identical telomeric and centromeric forms of SMN gene respectively.
• Both the SMN1 and SMN2 genes express SMN protein, however, the amount of functional full-length protein produced by SMN2 is much less than that produced by SMN1 due to the alternative splicing.
• The SMN gene is constitutively expressed in wide variety of tissues including brain, kidney, liver and spinal cord, while motor neurons are particularly vulnerable to reduced SMN protein levels. Deletion or mutational inactivation of the SMN1 gene causes spinal muscular atrophy (SMA), a lethal genetic disease characterized by loss of motor neurons in the spinal cord.
• The absence of SMN1 can be partially compensated for by SMN2 and the SMN2 expression level is associated with SMA severity. This kit is for the quantitative determination of SMN protein level in vivo.

Specifications

• Accession Number: NP_059107
• Assay Range: 62.5 to 4000 pg/mL
• Assay Sensitivity: 6 pg/mL
• Certifications/Compliance: ISO 13485
• Detection Method: Sandwich ELISA, Double Antibody
• Immunoassay Kit Format: Coated ELISA Kit
• Sample Type: Plasma, Serum
• Substrate: DAB
• Target Species: Human, Mouse
• Gene ID (Entrez): 6607
• Host Species: Human/Mouse
• Immunoassay Kit Grade: Solid
• Interassay CV: <10%
• Intraassay CV: <10%
• Shipping Condition: Blue ice
• Storage Requirements: -20°C to +4°C
• Total Assay Time: 3.5 hr.
• UniProt ID: Q16637

Safety and Handling

Product Identifier

• 96TEST Human B7-H3/CD276 ELISA Kit

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P234-Keep only in original container.
• P264-Wash skin thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P330-Rinse mouth.
• P362-Take off contaminated clothing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine