Invitrogen™ Human MMP-12 ELISA Kit

Human MMP-12 quantitates human MMP-12 in serum, plasma, supernatant.

Product Code	Brand	Quantity
17155099	Invitrogen™ EH327RBX5	5 x 96 Tests
17164623	Invitrogen™ EH327RB	96 Tests
18507854	Invitrogen™ EH327RBX10	10 x 96 Tests

Description

Human MMP-12 quantitates human MMP-12 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human MMP-12.

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22. 3.

Specifications

• Accession Number: 3.4.24.65; AV378681; HME; Macrophage elastase; Macrophage metalloelastase; macrophage-metalloelastase; matrix metallo protease; matrix metallopeptidase 12; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase); matrix metalloproteinase-12; ME; MGC138506; Mme; Mmel; MMP; MMP12; MMP-12; MMPs
• Assay Sensitivity: 0.04 ng/mL
• Product Type: ELISA
• Gene ID (Entrez): P39900
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: Research Use Only
• Target: MMP12
• Total Assay Time: 4 hr 45 min

• Assay Range: 0.04 to 10 ng/mL
• Conjugate: Biotin
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Mmp12
• Intraassay CV: <10%
• Label or Dye: HRP
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min