Invitrogen™ MIP-1b (CCL4) Human Instant ELISA™ Kit

For the quantitative detection of MIP-1beta levels in cell culture supernatants, serum and plasma.

Brand:  Invitrogen™ BMS2030INSTTEN

Product Code. 15561527

Includes: Aluminum pouch(es) with a Microwell Plate coated with Polyclonal Antibody to MIP-1beta, Biotin-Conjugate (anti-MIP-1beta polyclonal antibody), Streptavidin-HRP and Sample diluent, lyophilized
Aluminum pouch(es) with a human MIP-1beta Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Sample Diluent (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers

Description

The Human Macrophage Inflammatory Protein-1beta (CCL4)(Hu MIP-1β) ELISA quantitates Hu MIP-1β in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu MIP-1β. Principle of the method The Human MIP-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.

Specifications

• Accession Number: P13236
• Assay Sensitivity: 3.0 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CCL4
• Intraassay CV: 7.2%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 7.8-500 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 6351
• Interassay CV: 4.9%
• Kit Contents: Pre-coated 96 well Microplate, Adhesive Plate Covers, SAV-HRP, SAV-HRP, Sample Diluent, Standard curve strips, Wash Buffer Concentrate, Wash Buffer Concentrate, Stop Solution, Stop Solution, Lysis Buffer 10x
• Quantity: 10 x 128 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: CCL4, LAG-1, Small inducible cytokine A4, SCYA4
• Test Time: 20 min.

For Research Use Only