Invitrogen™ MBL Oligomer Human ELISA Kit

Product Code. 10202148

Brand: Invitrogen™ KIT029

Description

Sandwich ELISA Kit

The Human Mannose Binding Lectin (Hu MBL-2) ELISA quantitates Hu MBL-2 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu MBL-2. Principle of the method The Human MBL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Mannan-binding lectin (MBL; also called mannose binding lectin or protein) is a multimeric carbohydrate-binding protein produced in the liver and secreted into the blood, where it constitutes an important element in innate immune defense against invading microorganisms. Its normally oligomerized forms are associated with specific serine proproteases (the MASPs) which are activated when MBL binds to microbial carbohydrate surfaces and in turn activate complement via the MBL or lectin pathway. The determination of MBL concentrations in serum may be useful for the elucidation of suspected immune defects and as a prognostic indicator alerting to the need for heightened therapeutic or prophylactic measures in immunosuppressed patients, including patients receiving cancer chemotherapy, and patients with cystic fibrosis, SLE or rheumatoid arthritis.

Specifications

• Accession Number: P11226
• Assay Range: 0-40 ng/mL
• Assay Sensitivity: 5 pg/mL
• Description: Human MBL Oligomer ELISA Kit
• Conjugate: Biotin
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum
• Specificity: Western blotting of plasma or plasma fractions has not identified bands reacting with the antibody other than those attributable to MBL. The absence of cross-reaction with other plasma components is supported by the fact that readings indistinguishable fr
• Sufficient For: 96 microplate wells
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4153
• Gene Symbol: COLEC1,HSMBPC,MBLD,MBP,MBP-C,MBP1,MBPD,MBL2
• Intraassay CV: 5.5%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer concentrate, Chromogen, Stop Solution
• Label or Dye: HRP
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Precision: Undetermined
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma, 10 μL; Serum, 10 μL
• Storage Requirements: 2°C to 8°C
• Target: Human
• Test Time: 1 hr. 20 min.
• Total Assay Time: 3 hr. 15 min.