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Invitrogen™ Human MARCH4 (aa 312-396) Control Fragment Recombinant Protein

Product Code. 30197437
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Quantity:
100 μL
Unit Size:
100µL
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Product Code. 30197437

Brand: Invitrogen™ RP90983

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This item is not returnable. View return policy

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (79%), Rat (79%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-53149 (PA5-53149. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

RNF174 (MARCH4) is a multi-pass membrane protein mainly found in the membrane of Golgi apparatus. It is a complex enzyme with a RING-CH-type zinc finger. It acts as a ubiquitin-protein ligase mainly involved in third step of ubiquitin conjugation. The RINGv-type zinc finger domain is required for E3 ligase activity in which the E3 ubiquitin-protein ligase mediates ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. It is mainly expressed in brain and placenta.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q9P2E8
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 57574
Name Human MARCH4 (aa 312-396) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias BC056494; E3 ubiquitin-protein ligase MARCH4; Kiaa1399; MARCH4; MARCHF4; MARCH-IV; membrane associated ring finger 4; membrane associated ring-CH-type finger 4; membrane-associated ring finger (C3HC4) 4; membrane-associated ring finger (C3HC4) 4, E3 ubiquitin protein ligase; membrane-associated RING finger protein 4; Membrane-associated RING-CH protein IV; mKIAA1399; RING finger protein 174; RING-type E3 ubiquitin transferase MARCH4; RNF174
Common Name MARCH4
Gene Symbol MARCH4
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence WKVLNYDKTKDLEDQKAGGRTNPRTSSSTQANIPSSEEETAGTPAPEQGPAQAAGHPSGPLSHHHCAYTILHILSHLRPHEQRSP
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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