Invitrogen™ Human Legumain ELISA Kit

Product Code. 17135069

Brand: Invitrogen™ EH299RBX5

Description

ELISA

Human Legumain quantitates human Legumain in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Legumain.

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.

Specifications

• Accession Number: AEP; AI746452; Asparaginyl endopeptidase; AU022324; cysteine protease 1; legumain; LGMN; LGMN1; preprolegumain; protease, cysteine 1; protease, cysteine, 1; protease, cysteine, 1 (legumain); PRSC1; PRSC1EC 3.4.22.34
• Assay Range: 28.7 to 7,000 pg/mL
• Assay Sensitivity: 28.7 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5641
• Gene Symbol: LGMN
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: LGMN
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins